Contact us at
Contact us for more information about preventive measures and diagnostics leading to improved fish health and fish welfare: Info@patogen.no
Related videos
How to carry out tissue extraction for histology
In this video you will learn how to perform tissue sampling for histology. Histopathological examination of the full organ package including (1. Gill, 2.
Liver, 3. Pyloric caecae & pancreas, 4. Spleen, 5. Kidney, 6. Heart, 7. Skin and muscle) is the gold standard for assessing disease changes caused by
infectious and non-infectious causes. The order is not random and tissues must be fixed within 5 minutes after the fish is humanely euthanized. Using
anesthetics will not influence histopathological examinations, and can be used.
Step by step
- Cut off the second gill arch on the left side. Take care and do not touch the filaments with tweezers as they can harm the tissue
- Take out a small piece of kidney. Be careful to not squeeze sample with tweezers
- Take out a small piece of pyloric caecae (4-6 pieces)
- Take out a small part of the spleen. Be careful to not squeeze sample with tweezers
- Remove a small portion of the kidney just below the dorsal fin. Sample head and middle kidney
- Remove the heart by grasping the bulbus arteriosus. Gently cut off the atrium and make a small incision in the ventricle before fixation
- Sample a piece of skin/muscle over the lateral line organ to include skin, red and white skeletal muscle
- Lid on
- Beware of formalin, which is hazardous, and follow recommended health and security measures to avoid contact with hands, face and body parts.
Products used in the video
Pot with formalin
Sterile tweezer
Sterile scissors
Sterile scalpel blade
Business Hours
Switchboard open 08:00-15:00 (Scottish Time)
Urgent calls:15:00-20:00 (Scottish Time)
Laboratory is open all working days from 08:00-16:00
Business Hours Christmas/New Year 2024
Tuesday December 24th: Open 08:00-12:00
Wednesday December 25th: Closed
Thursday December 26th: Closed
Friday December 27th: Open 08:00-16:00
Monday December 30th: Open 08:00-16:00
Tuesday December 31st: Open 08:00-12:00
Exeptions for urgent calls: 24.12 and 31.12: 12:00-15:00
Special Business Hours 2025
Wednesday January 1st: Closed
Thursday January 2nd: Laboratory closed, reporting open due to Norwegian business hours
Wednesday April 17th: Laboratory Open 08:00-16:00 - reporting closed from 12:00 due to holiday in Norway
Thursday Apri 18thl: Laboratory Open 08:00-16:00 - reporting closed due to holiday in Norway
Friday April 20th: Closed
Monday May 5th: Laboratory Closed, reporting open due to Norwegian business hours
Monday May 26th: Laboratory Closed, reporting open due to Norwegian business hours
Monday August 4th: Laboratory Closed, reporting open due to Norwegian business hours
Monday December 1st: Laboratory Closed, reporting open due to Norwegian business hours
Wednesday December 24th: Open 08:00-12:00
Thursday December 25th: Closed
Friday December 26th: Closed
Wednesday December 31st: Open 08:00-12:00
 |  |
Rasmus Rønnebergs gt. 21, 6002 Ålesund, Norway
Copyright © 2023 PatoGen AS
