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How to perform sampling of blood and processing of serum
In this video you will learn how to perform sampling of blood and processing of serum. We have a panel of biomarkers related to different organs effected by various myopathies (PD, CMS and HSMI). We can also receive plasma.
Step by step
- Assembly the equipment as shown in the video.
- Aim the needle behind the caudal fin to aim for (Vena caudalis) and (Aorta caudalis) and stop when you hit the bone.
- Drag the handle on the Monovette back to create vacuum.
- Move the needle a bit back and forward and when you see blood in the Monovett, wait until it`s full.
- Remove the needle from the fish, put on the cap on the needle and invert the monovette x 3
- Before spinning the blood let the sample sit in upright position for a min of 20 min and spin within 2 hours post taking the sample
- Place Monovette in centrifuge and spin for 10 minutes at 6000rpm.
- Label tubes with sample and pen number.
- Carefully remove supernatants from blood tube with a pipette or carefully tilt the tube and allow the serum to enter the plastic tube.
- Make sure you label the samples sufficient and store the serum as required in fridge. Before sending the samples add a freezing pack to keep the samples cold during transport.
Products used in the video
Tube
Monovette
Needle
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